A method of purifying a target protein includes contacting a cell culture harvest or a protein preparation including at least one target protein with at least one fatty acid having 8 to 10 carbon atoms to form a mixture, contacting the mixture with one or more solids to form a mixture, the one or more solids comprise a cationic functional group, a metal binding functional group, or both, the metal binding functional group including a nitrogen-containing moiety selected from (1) a polyamine, (2) an imine, (3) an N-heterocycle, (4) an amino acid, (5) an N-hydroxyamide, (6), an arylamine, and combinations thereof, and separating solid materials after contacting the mixture with the one or more solids to provide a solution comprising the target protein.
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机译：纯化靶蛋白的方法包括使细胞培养物收获物或包含至少一种靶蛋白的蛋白制品与至少一种具有8至10个碳原子的脂肪酸接触以形成混合物，使该混合物与一种或多种固体接触以形成在混合物中，一种或多种固体包含阳离子官能团，金属结合官能团或两者，金属结合官能团包括选自以下的含氮部分：（1）多胺，（2）亚胺，（3 ） N -杂环，（4）氨基酸，（5） N -羟酰胺，（6），芳基胺及其组合，并分离固体物质在使混合物与一种或多种固体接触以提供包含靶蛋白的溶液之后。
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