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IRF-3 Antibody (SL-12) - Western Blotting - Image 394363

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IRF-3 Antibody (SL-12) HRP: sc-33641 HRP. Direct western blot analysis of IRF-3 expression in HL-60 (A), HT-1080 (B), U-937 (C), HeLa (D), K-562 (E) and MCF7 (F) whole cell lysates.

IRF3 抗体 (SL-12): sc-33641

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说明书
  • IRF3 抗体 SL-12 是小鼠单克隆 IgG1 κ，IRF3抗体, 在76篇文献中引用，规格为200 µg/ml
  • raised against recombinant IRF-3 fusion protein corresponding to human IRF-3 (amino acids 56-427)
  • IRF3 抗体 (SL-12) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 IRF-3
  • 抗-IRF3 抗体 (SL-12) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-33641 X, 200 µg/0.1 ml)
  • 关于如何获取IRF3 (SL-12): sc-33641的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP and m-IgG1 BP-HRP 是 IRF3 Antibody (SL-12) for WB and IHC(P)首选应用二抗。这些试剂现在以套装形式提供IRF3 Antibody (SL-12) (见下方订购信息).

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IRF-3 Antibody (SL-12) is an IgG1 κ mouse monoclonal IRF-3 antibody (also designated IRF3 antibody) that detects the IRF-3 protein of mouse, rat and human origin by WB, IP, IF and IHC(P). IRF-3 Antibody (SL-12) is available as both the non-conjugated anti-IRF-3 antibody form, as well as multiple conjugated forms of anti-IRF-3 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Interferon regulatory factor-1 (IRF-1) and IRF-2 have been identified as novel DNA-binding factors that function as regulators of both type I interferon (interferon-α and β) and interferon-inducible genes. The two factors are structurally related, particularly in their N-terminal regions, which confer DNA binding specificity. In addition, both bind to the same sequence within the promoters of interferon-α and interferon-β genes. IRF-1 functions as an activator of interferon transcription, while IRF-2 binds to the same cis elements and represses IRF-1 action. IRF-1 and IRF-2 have been reported to act in a mutually antagonistic manner in regulating cell growth; overexpression of the repressor IRF-2 leads to cell transformation while concomitant overexpression of IRF-1 causes reversion. IRF-1 and IRF-2 are members of a larger family of DNA binding proteins that includes IRF-3, IRF-4, IRF-5, IRF-6, IRF-7, ISGF-3γ p48 and IFN consensus sequence-binding protein (ICSBP).

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Is this antibody able to detect IRF3 dimers ?

Asked by: LeeL
Thank you for your question. We have not detected IRF-3 dimers with this antibody, however, it's possible that the antibody could detect a dimer if it was present in the sample.
Answered by: Tech Service
Date published: 2020-06-01

可以染小鼠组织免疫荧光吗

Asked by: 123mmmm
Thanks for your question. IRF-3 (SL-12) is recommended for detection by IF on mouse origin. 感谢您的问题。IRF-3 抗体 (SL-12)推荐免疫荧光方法检测小鼠来源的样本。
Answered by: Technical Support 9
Date published: 2020-05-19

You report that sc-33641 detects IRF3 of mouse and human origin, yet all your examples are human. Do you have an example showing positive WB in mouse cells/tissue, with verification it is NOT present in a KO or is induced in a TG?

Asked by: jjmaher
Thank you for your question. We do not have data in mouse cells or tissues at this time. The antibody is recommended for detection of IRF-3 of mouse, rat and human origin based on sequence identity. If the antibody does not work in any of the recommended applications or species, please contact our Technical Service Department and we would be happy to provide you with a complimentary replacement antibody, refund or credit per our product warranty.
Answered by: Technical Support
Date published: 2021-08-09

When should the TransCruz reagent sc-33641 X be used instead of the standard form sc-33641?

Asked by: jenniferc
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support 5
Date published: 2017-01-20
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Rated 1 out of 5 by Cinzia from Not so good in IF For us this ab didn't worked in IF on LLC-MK2 (dil 1:100)
Date published: 2023-06-29
Rated 5 out of 5 by a20220522 from 功能很出色的 抗体 多次使用，效果优秀，多次反复使用都没有问题，强于其他公司的抗体，值得大家购买和推荐。1：1500都可以做出来。多个实验都可以做出比较理想的效果。值得推荐。赞一个。
Date published: 2022-05-22
Rated 3 out of 5 by Anonymous from IF (free sample) used for IF for characterization of tumor cells, No problem for signal detection
Date published: 2020-10-06
Rated 5 out of 5 by daotongyi from very beautiful WB I get prospective results by using this IRF3 antibody!
Date published: 2017-12-17
Rated 5 out of 5 by Stefanie Kretschmer from Very strong signal in WB Shows a very strong signal in WB at a dilution of 1:200 (10 ug protein loaded per lane)
Date published: 2017-10-18
Rated 5 out of 5 by 亚路井1 from work well we got it several months ago and it works well, we like it !
Date published: 2017-10-11
Rated 5 out of 5 by anonym from IRF3 Works great in Western Blot. Used in 5% milk powder.
Date published: 2017-10-10
Rated 4 out of 5 by helix from Why I can't see the nuclear translocation of IRF3? I can detect signal. But can't see any nuclear translocation for IRF3 after poly(I:C) stimulation for macrophage. I don't know why, the antibody I used for IF is 1:250.
Date published: 2017-09-19
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IRF3抗体 (SL-12) is rated 4.6 out of 5 by 20.
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