Ohio State University, OARDC 1 article published in JoVE Chemistry Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds Michael Joe Vaughan1,2, Ann Chanon3, Joshua J. Blakeslee1,3,4 1Center for Applied Plant Sciences, The Ohio State University, OARDC, 2Department of Plant Pathology, The Ohio State University, OARDC, 3Department of Horticulture and Crop Science, The Ohio State University, OARDC, 4OARDC Metabolite Analysis Cluster, The Ohio State University, OARDC This article presents a method for the detection and quantification of organic acids from plant material using free zonal capillary electrophoresis. An example of the potential application of this method, determining the effects of a secondary fermentation on organic acid levels in coffee seeds, is provided.
