Summary
The distribution of tyrosinated and detyrosinated tubulin in microtubule arrays of pine and onion cells was investigated by immunofluorescence techniques. Staining of isolated cells and methacrylate sections ofPinus radiata andAllium cepa root tips indicated that all microtubule structures contained tyrosinated tubulin but not the posttranslationally modified detyrosinated tubulin. The detyrosinated tubulin epitope was, however, created in vitro by treating both sections and fixed whole cells with carboxypeptidase A.
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Gilmer, S., Clay, P., MacRae, T.H. et al. Tyrosinated, but not detyrosinated, α-tubulin is present in root tip cells. Protoplasma 210, 92–98 (1999). https://doi.org/10.1007/BF01314959
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DOI: https://doi.org/10.1007/BF01314959