Abstract
The aim of this work was to create the new biosensor of alanine transaminase (ALT) activity detection in water samples. A platinum disk electrode was used to transduce chemical reactions into amperometry measured signal. Nanosize polyphenylendiamine (PPD) was applied to improve the selectivity of the transducer. In the work, the most effective isomer PD was selected and the stability of the additional membrane based on it was checked. The bioselective element was formed by the immobilization of glutamate oxidase on the surface of the transducer covered by a semipermeable P-meta-PD membrane. There was reported the method of ALT activity measurement. There was also reported the optimal conditions to create this biosensor (including enzyme immobilization parameters and substrates concentrations). The analytical characteristics were investigated including the sensitivity, the linear range, the minimum limit of detection, time of measurement, duration of analysis, stability, etc. Also, there was explored the biosensor selectivity to different interferents, e.g., electroactive molecules and amino acids. For the purpose to test the fundamental possibility of this biosensor to measure the ALT activity in real samples, the operation of the biosensor was tested in a solution with an ionic composition close to serum.
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Data Availability
Raw data and derived data supporting the findings of this study are available from the corresponding author Mruga D. on request.
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Acknowledgements
The work was carried out at the expense of a grant of the National Academy of Sciences of Ukraine to research laboratories of young scientists of the NASU for conducting research in the priority directions of the development of science and technology in 2022–2023.
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Mruga, D., Dzyadevych, S. & Soldatkin, O. High-selective alanine transaminase-sensitive biosensor based on nanosize semipermeable poly-meta-phenylenediamine membrane. Appl Nanosci 13, 6939–6949 (2023). https://doi.org/10.1007/s13204-023-02810-9
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DOI: https://doi.org/10.1007/s13204-023-02810-9