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effect of introducing a disulphide bond between the a and c domains on the activity and stability of
M. On the other hand, the IC50 of Sfamy02 for EDTA was 0.45 mM,nearly two times lower than that of Sfamy01 at 0.77 mM. These results show that the introduction ofa disulphide bridge had little effect on the enzyme activity, but made the enzyme more susceptible tocalcium ion extraction. Altogether, the new disulphide bridge improved the enzyme...
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